NEWS

30. April 2013
tetraProbes for Fluorescence in Situ Hybridization (FISH)

See more! With the biomers.net tetraProbes. Brighter than mono-labelled or dual-labelled hybridisation probes.

• Highest sensitivity due to 4 labels per oligonucleotide
• Fluorescein / Fam, Cy3, Cy5, ATTO- and Dyomics-dyes
• Nearly no influence on the hybridization properties as the dyes are coupled to the oligonucleotide backbone
• Special probe-design prevents "self-quenching effects"

See for yourself and test the biomers.net tetraProbes. Please contact our customer support for ordering a sample of EUB338-probe (Fam or Cy3 label, delivery amount: 1 OD, special offer price : 79,00 EUR / 71,10 GBP).

16. October 2012
New: LightCycler dye CYAN 500

Now available for online ordering: Oligonucleotides with the LightCycler dye Cyan 500 as 5' modification; also available in combination with a 3'-quencher DDQ I (TaqMan probes) or 3'-Phosphat (hybridization probes). 

Optical properties of Cyan 500: Abs. 436 nm, Em. 481 nm

05. July 2012
2'-fluoro-RNA oligos, UPDATE

2'-fluoro modified RNA-oligos with additional 5´-phosphorilation as potential RNAi-Tools:

"mRNA knockdown by single strand RNA is improved by chemical modifications"; Haringsma H.J., Li J.J., Soriano F., Kenski D.M., Flanagan W.M., Willingham A.T., Nucleic Acids Research 2012, 40, 4125-4136

Our Offer: Full-2'-F-RNA-oligos with 18 - 27 bases on Scale FM27.

[More]

27. June 2012
Replacements for VIC, NED, PET, LIZ

Yes, they exist, the substitutes for ABI dyes:

19.06.2012
Copper-free click chemistry

5´-DBCO-TEG modifications
The alternative to copper catalyzed azide-alkyne-cycloaddition, without any toxic metals; suitable for click chemistry under physiological conditions.

Do not hesitate to contact us. We will find a solution for your click projects.
[More]

19.06.2012
5-Propynyl-Pyrimidines

Increased specificity through higher duplex stability.
The replacement of "normal" pyrimidine bases by 5-propynyl-pyrimidine building blocks will lead to higher melting temperatures.

dC replaced by pdC: up to + 2,8 °C per exchange

dT replaced by pdU: up to + 1,7 °C per exchange

Improvement of hybridization properties in e.g. antisense, FISH and Real-time PCR experiments.
biomers.net offers oligonucleotides and probes with 5-propynyl-pyrimidines for research purposes only.
[More]

01.06.2012
Oligonucleotides with synthesis scale XS

special offer!
50% discount for all oligonucleotides with synthesis scale XS.

- all oligos are purified by reverse phase cartridge.
- quality control by MALDI mass spectrometry for all oligos up to 50 bases.
- guaranteed amount >2 OD.
- 12 to max. 60 bases, no modifications, no wobbles

(This offer is valid until Sept. 30th, 2012 for all online orders from Great Britain. Additional conditions are according to our GBP pricelist. Prices may change without further notice. Errors excluded. The discount price listed above cannot be combined with other discounts apart from the “Early Bird Discount“.)

31.05.2012
FAM/TQ2 Real-Time-PCR Probes

Our special offer price for 6-Fam/TQ2 probes:

79,00 EUR / 71,10 GBP (Synthesis scale S, HPLC purification included, max. length of 40 bases).

Tide Quencher dark acceptors are a series of quenchers which are optimized to pair with a broad range of classic fluorophores. Tide Quencher acceptor dyes exhibit comparable or even better performance than other commonly used quenchers and are license-free.

TQ2 and TQ3 real-time PCR probes are now available for online ordering.

If you require a dye/quencher combination which is not listed in the online order form, please do not hesitate to contact us.

(This offer is valid until Dec. 31st, 2012 for all online orders from Great Britain. VAT and shipping costs may apply. Additional conditions are according to our current pricelist. Prices may change without further notice. Errors excluded. The discount price listed above cannot be combined with other discounts apart from the “Early Bird Discount“.)

28.02.2012
The new XXL-SCALE
for oligonucleotide amounts from 25 to 500 mg is now available.                                                                          
More Details.                                                                                                                                                                            

28.02.2012
Desthiobiotin modified oligonucleotides!

Desthiobiotin - a biotin analogue lacking a sulfur atom - binds to streptavidin like biotin but with significantly lower affinity. Accordingly, captured oligonucleotides /nucleic acids  labeled with destiobiotin can easily displaced from surfaces or beads  by addition of buffered biotin solution or incubation at 95 degree C. 5' modified oligonucelotides with desthiobiotin are available in synthesis scales S, M, and L. For any additional information, please contact us.

Literature:
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation, Hirsch, J.D., Eslamizar, L., Filanoski, B.J., Malekzadeh, N., Haugland, R.P., Beechem, J.M., Haugland, R.P. , Anal. Biochem. (2002), 308: 343-357.
Robust Definition and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay, van Doom, R., Slawiak, M., Szemes, M., Dullemans, A.M., Bonants, P., Kowalchuk, G.A., Schoen, C.D., Appl. Environ. Microbiol. (2009), 75: 4185-4193.
Reversible Association/Dissociation Reaction of Avidin on the Dendrimer Monolayer Functionalized with a Biotin Analogue for a Regenerable Affinity-Sensing Surface, Yoon, H.C.; Hong, M.-Y.; Kim, H.-S., Langmuir (2001), 17: 1234-1239.

14.09.2011
Oligonucleotides for DOPE-FISH

Several months ago, Stoecker et al. systematically evaluated the effect of 3’,5’ double labeled oligonucleotides on the fluorescence signal intensity in in situ hybridization experiments in comparison to single labeled probes. They describe a doubling of signal intensity without loss of specificity. In these experiments probes with the following labels have been tested: FLUOS (= Fluorescein, FAM, 6-FAM), Cy3 and Cy5.

Double labeled probes (6-Fam, Cy3, Cy5) for DOPE-FISH experiments are available for online ordering.

Literature:
Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility, Stoecker K, Dorninger C, Daims H, Wagner M; Appl. Environ. Microbiol. 76, 922-926 (2010)

12.09.2011
SIMA, an alternative dye to Hex

SIMA (4,7-Dichloro-2′,7′-diphenyl-fluorescein) provides an excellent alternative to Hex. The absorption and emission maxima of SIMA are virtually identical to Hex, thus SIMA is a full replacement of Hex in nearly all fluorescent applications. The absorption band is broader and the extinction coefficient is smaller than that of Hex, however, when excited at 488 nm a dT20 oligonucleotide with 5′-SIMA exhibits the same fluorescence intensity as the corresponding oligo labeled with Hex.

Furthermore, SIMA is chemically more stable than Hex, which reduces degradation and formation of by-products during oligonucleotide synthesis and deprotection and thus makes the purification of labeled oligonucleotides easier.

Oligonucleotides with 5′-SIMA label are available from biomers.net as well as SIMA/BHQ-1 and SIMA/TQ2 qPCR probes.

15.07.2011
Deep Dark Quencher Probes

Real Time PCR-Probes with Deep Dark Quencher: Fam/DDQ I  at a special price - more details: here
And now additionally: DDQ II, optimal in combination withCy3, Cy5, Cy5.5, California Red, Sunnyvale Red oder Tamra - more details: here.

11. April 2011
LightCycler-Probes with biomers.net

With the LightCycler^®-assay two probes are placed on the target-DNA, one behind the other. Due to the required binding of both probes, the specificity of such assays is extremly high, enabling a differentiation of closely related genes or organisms.
The 5'-located probe (donor-probe) bears a fluorophore at the 3'-end, whose fluorescence is not directly detected, but used to excite the second probe in close proximity. This light transfer by FRET (fluorecsence resonance energy transfer) ensures that a result is obtained only upon proper binding of both probes.

biomers.net now offers both 3'-6-Fam and 3'-FITC for the donor probe! Read more.

17.01.2011
News for Specialists

Left-turning helices can now be generated with particular building blocks. Find more information here. 

17.01.2011
Transporter through membranes: Cholesterol and now NEW!! Tocopherol

Cholesterol and Tocopherol coupled to nucleic acids enable an easy passage through cell membranes. Read more here!

11. Jan.2011
...in red!

New dyes in the red range! Try the new alternatives to Rox and Texas Red! Find here further information.

24.Sep.2010
Blocker-Oligos

Oligos can do more than just hybridise! How an oligo will act as a stop-sign, read here.