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The Instant Quencher 4 (IQ4) with an absorption maximum of 587 nm (quenching range 500-700 nm) is well suited as an alternative for BHQ-2.
The quencher for the range of 400-530 nm, e.g. Atto 425/BMN-Q460 or Atto 465/BMN-Q460.
- Smart-Seq for transcriptome analysis (Picelli et al., 2014)- Mediator Probe PCR (Faltin et al., 2012)
Increased qPCR sensitivity with DoubleQuenched Probes.