Scorpions

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New at biomers.net: SCORPIONS^®

biomers.net now offers also Scorpions-Oligos.

Scorpions-primer are multiple modified oligonucleotides which are used in a particular variant of Real-time PCR to generate highly specific signals.

Scorpions-oligos bear at their 5´-end a fluorophore, which in the unhybridised state is kept in close proximity to a quencher by a particularly designed stem structure. Further 3', following the quencher, a target-specific primer is attached. This in combination with an unmodified 'reverse' pimer is used in a PCR reaction to amplify the target region. To prevent the polymerase from filling up the complete Scorpions-sequence, a further modification is included, leading to a stop of polymerisation near the quencher, thus, the loop/stem-region of the Scorpions-oligo will remain as single-strand.

In a Scorpions-PCR the basic events are as follows:

Initially the primer sequence of the Scorpions-oligo will anneal to its respective target sequence. After the primer has been elongated, the stem structure will open and compete with the binding of the newly synthesised strand.

The opening of the stem enables the fluorophore to escape the influence of the quencher, thus, the fluorescence now can be used for detection.

biomers.net offers Scorpions at the following conditions:

Synthesis Scale			S	M	L
Yield (HPLC)			> 1OD	>2 OD	> 4 OD
Dye	Quencher	Blocker
FAM, HEX, TET	int. BHQ-1	Spacer 18	EUR 290,00 CHF 469,80 GBP 232,00	EUR 390,00 CHF 631,80 GBP 312,00	EUR 520,00 CHF 842,40 GBP 416,00
Cy3, Cy5	int. BHQ-2	Spacer 18	EUR 290,00 CHF 469,80 GBP 232,00	EUR 390,00 CHF 631,80 GBP 312,00	EUR 520,00 CHF 842,40 GBP 416,00
FAM, HEX, TET	int. Dabcyl	Spacer 18	EUR 270,00 CHF 437,40 GBP 216,00	EUR 370,00 CHF 599,40 GBP 296,00	EUR 500,00 CHF 810,00 GBP 400,00
Cy3, Cy5	int. Dabcyl	Spacer 18	EUR 270,00 CHF 437,40 GBP 216,00	EUR 370,00 CHF 599,40 GBP 296,00	EUR 500,00 CHF 810,00 GBP 400,00
further combinations on request

Scorpions are produced with a licence from DxS.
Prices are inclusive DNA-oligonucleotide up to 60 bases, 5´-fluorophore, internal coupling of the quencher as well as the polymerase blocker, HPLC-purification and MALDI quality control and are valid only for electronic ordering.