NOVEDADES

16. October 2012
New: LightCycler dye CYAN 500

Now available for online ordering: Oligonucleotides with the LightCycler dye Cyan 500 as 5' modification; also available in combination with a 3'-quencher DDQ I (TaqMan probes) or 3'-Phosphat (hybridization probes).

Optical properties of Cyan 500: Abs. 436 nm, Em. 481 nm

23. July 2012
tetraProbes for Fluorescence in Situ Hybridization (FISH)


See more! With the biomers.net tetraProbes. Brighter than mono-labelled or dual-labelled hybridisation probes.

• Highest sensitivity due to 4 labels per oligonucleotide
• Fluorescein / Fam, Cy3, Cy5, ATTO- and Dyomics-dyes
• Nearly no influence on the hybridization properties as the dyes are coupled to the oligonucleotide backbone
• Special probe-design prevents "self-quenching effects"

See for yourself and test the biomers.net tetraProbes. Please contact our customer support for ordering a free sample of EUB338-probe (Fam).

05. July 2012
2'-fluoro-RNA oligos, UPDATE

2'-fluoro modified RNA-oligos with additional 5´-phosphorilation as potential RNAi-Tools:

"mRNA knockdown by single strand RNA is improved by chemical modifications"; Haringsma H.J., Li J.J., Soriano F., Kenski D.M., Flanagan W.M., Willingham A.T., Nucleic Acids Research 2012, 40, 4125-4136

Our Offer: Full-2'-F-RNA-oligos with 18 - 27 bases on Scale FM27.

[More]

27. June 2012
Replacements for VIC, NED, PET, LIZ

Yes, they exist, the substitutes for ABI dyes:

19.06.2012
Copper-free click chemistry

5´-DBCO-TEG modifications
The alternative to copper catalyzed azide-alkyne-cycloaddition, without any toxic metals; suitable for click chemistry under physiological conditions.

Do not hesitate to contact us. We will find a solution for your click projects.
[More]

19.06.2012
5-Propynyl-Pyrimidines


Increased specificity through higher duplex stability.
The replacement of "normal" pyrimidine bases by 5-propynyl-pyrimidine building blocks will lead to higher melting temperatures.

dC replaced by pdC: up to + 2,8 °C per exchange

dT replaced by pdU: up to + 1,7 °C per exchange

Improvement of hybridization properties in e.g. antisense, FISH and Real-time PCR experiments.
biomers.net offers oligonucleotides and probes with 5-propynyl-pyrimidines for research purposes only.

[More]

28.02.2012
Desthiobiotin modified oligonucleotides!

Desthiobiotin - a biotin analogue lacking a sulfur atom - binds to streptavidin like biotin but with significantly lower affinity. Accordingly, captured oligonucleotides /nucleic acids  labeled with destiobiotin can easily displaced from surfaces or beads  by addition of buffered biotin solution or incubation at 95 degree C. 5' modified oligonucelotides with desthiobiotin are available in synthesis scales S, M, and L. For any additional information, please contact us.

Literature:
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation, Hirsch, J.D., Eslamizar, L., Filanoski, B.J., Malekzadeh, N., Haugland, R.P., Beechem, J.M., Haugland, R.P. , Anal. Biochem. (2002), 308: 343-357.
Robust Definition and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay, van Doom, R., Slawiak, M., Szemes, M., Dullemans, A.M., Bonants, P., Kowalchuk, G.A., Schoen, C.D., Appl. Environ. Microbiol. (2009), 75: 4185-4193.
Reversible Association/Dissociation Reaction of Avidin on the Dendrimer Monolayer Functionalized with a Biotin Analogue for a Regenerable Affinity-Sensing Surface, Yoon, H.C.; Hong, M.-Y.; Kim, H.-S., Langmuir (2001), 17: 1234-1239.

12.09.2011
SIMA, an alternative dye to Hex

SIMA (4,7-Dichloro-2′,7′-diphenyl-fluorescein) provides an excellent alternative to Hex. The absorption and emission maxima of SIMA are virtually identical to Hex, thus SIMA is a full replacement of Hex in nearly all fluorescent applications. The absorption band is broader and the extinction coefficient is smaller than that of Hex, however, when excited at 488 nm a dT20 oligonucleotide with 5′-SIMA exhibits the same fluorescence intensity as the corresponding oligo labeled with Hex.

Furthermore, SIMA is chemically more stable than Hex, which reduces degradation and formation of by-products during oligonucleotide synthesis and deprotection and thus makes the purification of labeled oligonucleotides easier.

Oligonucleotides with 5′-SIMA label are available from biomers.net as well as SIMA/BHQ-1 and SIMA/TQ2 qPCR probes.

11. April 2011
LightCycler-Probes with biomers.net

With the LightCycler^®-assay two probes are placed on the target-DNA, one behind the other. Due to the required binding of both probes, the specificity of such assays is extremly high, enabling a differentiation of closely related genes or organisms.
The 5'-located probe (donor-probe) bears a fluorophore at the 3'-end, whose fluorescence is not directly detected, but used to excite the second probe in close proximity. This light transfer by FRET (fluorecsence resonance energy transfer) ensures that a result is obtained only upon proper binding of both probes.

biomers.net now offers both 3'-6-Fam and 3'-FITC for the donor probe! Read more.

17. Enero 2011
L-ADN!

biomers.net ofrece componentes especiales de L-ADN, para manipulación del sentido de rotación de una hélice
hacia la izquierda. Le ofrecemos las siguientes modificaciones para la construcción interna aquí.

17. Enero 2011
Tocoferol y colesterol

Nuevo! Acoplamientos de colesterol y tocoferol (vitamina E) a ácidos nucleicos como una confiable "ayuda-transporte"
a través de membranas. Pinche aquí para ver más información.

17. Enero 2011
Tapas de Estilbeno: Mayor estabilidad del duplex

La tapa de 5’-trimetoxi estilbeno, permite un aumento en el punto de fusión o a una mayor estabilidad del duplex,
posibilitando una mejor dicriminación de mismatch.
A partir de ahora están disponibles en biomers.net: oligonucleótidos con 5‘-Trimetoxi Estilbeno!

04.Nov.2010
Yakima Yellow!

biomers.net ofrece a partir de ahora  Yakima Yellow! Lea más detalles aquí.                

03.Sept.2010
LA modificación para epigenética: 5-Me-dC

La metilación de citidinia cumple un rol muy importante en la organización y actividad de la información genética de una célula. biomers.net ofrece 5-Me-dC como acoplamiento interno a un oligonucleótido hasta un largo máximo de 140 bases.

04. Sept. 2009
Alternativa para Hex

Nuevo en biomers.net: Un colorante alternativo para Hex! SIMA posee propiedades casi idénticas como Hex, más detalles aquí.