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The oligonucleotide 6-Fam-dArUdAdA-BHQ1 is used as a substrate for a convenient proof of RNases.
In inactivated state the quencher causes full extinction of the fluorescence of fluorescein. After digestion by the RNase, however, quencher and fluorophore are separated from each other and thus the fluorescence of the 6-Fam reporter can be detected at 520 nm.
|Increase of fluorescence induced by digestion of the substrate (200 pM RNase A, 100 nM RNase substrats, 100 mM MES buffer, pH= 6.0, 100 mM NaCl), detection at 515 nm, excitation at 490 nm||Fluorescene spectrum of the cleaved substrate|
6-Fam-dArUdAdA-BHQ1: RNase substrate
Quantity delivered: 10 nmol (lyophilised)
Price: 195,00 € (VAT and shipping costs may apply)
We will also gladly synthesise different reporter-quencher-systems for you. Feel free to contact us and ask for a quote
B. R. Kelemen, T. A. Klink, M. A. Behlke, S .R. Eubanks, P .A. Leland and R. T. Raines. Hypersensitive substrate for ribonucleases. Nuc. Acids Chem. 1999, 27, 3696-3701
L. E. Bretscher, R. L. Abel and R. T. Raines. A Ribonuclease Variant with Low Catalytic Activity but High Cytotoxicity. J. biol. Chem. 2000, 275, 9893-9896