Page id: 209

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The addition of a C3 spacers (3 hydrocarbons) to the 3´-end of an oligonucleotide can prevent e.g. its elongation during a PCR without influencing its annealing properties in any meaningful way. Such a modification is therefore well suited for hybridization probes used in a PCR reaction.

biomers.net offers different terminal and internal spacers:

  • Spacer C3, Spacer C6, Spacer C12
  • Spacer9, Spacer12, Spacer18
  • dSpacer
      Modification                5´                 3´                        internal
           Spacer C3 x x x
           Spacer C6 x x x
           Spacer C12 x - x
           Spacer9 x - x
           Spacer12 x - x
           Spacer18 x x x
           dSpacer x - x
 

   terminal and internal Spacer