BMN Dyes and Quenchers
BMN Dyes– Dyes from biomers.net
BMN-488, BMN-505, BMN-536, BMN-562
In addition to the wide range of known fluorescent dyes, biomers.net also offers its own excellent alternatives.
The so-called BMN dyes can be attached to an oligonucleotide at the 5´- or 3´-end.
|Dye||ABS [nm]||EM [nm]||Alternative to|
|BMN-488||495||519||Atto 488, Alexa Fluor 488® (identical structure)|
|BMN-505||507||524||Fam, Fluorescein, FITC|
|BMN-536||536||553||Carboxyrhodamin 6G, Atto Rho6G|
BMN Quencher – Quenchers from biomers.net
BMN-Q460, BMN-Q535, BMN-Q590, BMN-Q620, BMN-Q650
In addition to this wide range of available quenchers, biomers.net also offers a "proprietary" quencher set.
The BMN quenchers of biomers.net are excellent alternatives to the traditional Dark Quenchers due to their optimal matching quenching range.
|Quencher||ABS [nm]||Quenching Range [nm]||Alternative to|
|BMN-Q460||453||400-530||Dabcyl, DDQ I|
|BMN-Q535||494||480-580||BHQ-1, TQ2, DDQ I, Iowa Black FQ, Eclipse-Quencher, Dabcyl|
|BMN-Q620||550||480-720||BHQ-2, TQ3, BBQ-650, DDQ II, Iowa Black RQ|
|BMN-Q650||554||550-720||BBQ-650, DDQ II|
Figure1: dT20 oligo with 3´-quencher, meaurement of absorption spectra in buffer (pH 7.0)
With an absorption maximum of 460 nm, our Dark Quencher BMN-Q460 is particularly suitable for the short-wavelength region and represents an excellent alternative for the quenchers Dabcyl and DDQ I.
In addition to use in Molecular Beacons, in which it inhibits fluorescent signals by contact-quenching, BMN-Q460 can be used in various molecular biological applications. As 3´-quencher in Dual-Labelled probes (5´-nuclease assay), BMN-Q460 can be used in the range of 400-530 nm, so it is ideally suited for the fluorophores Atto 390, Atto 425 and Atto 465.
biomers.net offers the quencher BMN-Q460 as 5´-, 3´- or internal modification.
BMN-Q535 is the second Dark Quencher of biomers.net and has an absorption maximum at about 535 nm and a quenching range from about 480 nm to 580 nm. This is the same range as BHQ-1, TQ2, DDQ I, Eclipse-Quencher or Iowa Black FQ, so BMN-Q535 may be an excellent alternative for this quenching.
BMN-Q535 is used as 3'-modification for standard Dual-Labelled Probes and Molecular Beacons.
We offer this quencher as 5 'or 3 modification. In addition, BMN-Q535 can be used as internal modification in Double Quenched probes with increased sensitivity.
|Dual-Labelled Probe||Double Quenched Probe||Molecular Beacon Probe|
As an ideal alternative for BHQ-2, the dark quencher BMN-Q590 is well suitable for fluorescent dyes like Tamra, Cyanine 3, Rox or Cyanine 3.5. It has an absorption maximum of 590 nm and a quenching range of approx.
550-650 nm and can be ordered as 3´-modification.
Similar to the quenchers BMN-Q460 and BMN-Q535, biomers.net also offers the dark quencher BMN-Q620 as
5´, 3´ and internal modification. Thus, BMN-Q620 can be used ideally in Double Quenched Probes for the longer wavelength region. With an absorption maximum of 620 nm and a quenching range of 480 nm to 720 nm, it has a relatively wide range and can therefore optimally quench fluorescence dyes even in short-wave range.
With its absorption maximum of 650 nm and a quenching range of 550-720 nm, BMN-Q650 ideally covers the long-wavelength region and can be used as an alternative to BBQ-650 oder DDQ II.
BMN-Q650 is available as 3´-modification.