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Cysteine (tbuSS) (3' modification)
The aminothiol modification cysteine reacts with cyanobenzothiazole and can effectively combine different biomolecules. Cysteine is supplied in a protected form (cysteine with StBu protecting group) and needs to be treated with an appropriate reducing agent (e.g. TCEP) before further conjugation steps, in order to reduce the disulfide and release the thiol group. The TCEP can be added, for example, to the conjugation buffer.