6-Fam (Internal modification)
Molecular weight [Da]
The dye 6-Fam as well as 5-Fam is an isomer of fluorescein. Both dyes differ only by the position of the linker (C5 or C6). Fam is one of the most commonly used dyes and can be used as label for direct fluorescence imaging or haptene-label that can be visualized with immuno-detection linked either to chemiluminiscence or colour formation.
Here, the internal modification can be attached via an amino-dC or -dT building block.
Chemical structure of dT-Fam bound to an oligonucleotide